Proteomics Platform

Proteomics Platform

  • Proteomics of whole organisms, organs, organelles, tissues, cell pellets, pulldowns, complex or single protein mixes from solutions or gels
  • Quantification by label-free or multiplexed labeling (e.g., TMT-10/16plex) techniques with microscale sub-fractionation to increase coverage
  • High resolution/high mass accuracy data for intact mass protein analysis
  • Phosphoproteomics using TiO2 phosphopeptide enrichment
  • Technological platforms supported by bioinformatics tools to process data and report results

  • Post-translational modification characterization

     


Thermo Orbitrap Astral with FAMIS coupled to Vanquish Neo UHPLC
Laboratory Thermo Orbitrap Astral with FAMIS coupled to Vanquish Neo UHPLC sitting in a lab
Thermo Orbitrap Astral with FAMIS coupled to Vanquish Neo UHPLC
  • MS2 acquisition rates up to 200 Hz
  • dynamic range >1,000
  • standard mass range of m/z 40–6,000
     

 

Thermo Q-Exactive – High Field (QE-HF) coupled to Thermo Vanquish Flex UPLC
  • High Resolution/Accurate Mass (HR/AM) instrument
  • Resolving power up to 240,000 at 200 m/z
  • Sub ppm mass accuracy
  • Scan speed of 18 Hz at 15,000 resolution
  • Flow rate of the nanopump: 20 nL/min to 1.5 µL/min
  • Pressure to 800 bar (11,600 psi)
Laboratory bench setup featuring a Thermo Scientific Q‑Exactive mass spectrometer connected to liquid chromatography components. The image shows stacked analytical modules with solvent bottles on top and a mass spectrometer unit to the right. In the foreground, gloved hands hold a sample tray being loaded into the instrument. Cabinets, tubing, and a computer monitor are visible, showing an active high‑resolution mass spectrometry used for proteomics or metabolomics analysis.
Thermo Q-Exactive

 

❖ Mascot 2.6.1 (Matrix Science Ltd) for protein identification from MS/MS spectra

❖ Proteome Discoverer 2.4 Search Engine (Thermo) for metabolic, isotope labeling and label free quantitative proteomics

❖ Scaffold 4.8.9 (Proteome Software) for protein identification, reporting and label-free quantitative proteomics

❖ PEAKS 8 (Bioinformatics Solutions Inc) for de novo sequencing and PTM characterization