Full Services - Metabolomics

Full Services User Overview

A virtual consultation with the PMF Director is required prior to submitting samples to check for compatibility of sample type and format with our analytical approaches and to confirm the experimental approach and expectations for the services. Please review the Full Service Users section of our Policies and Procedures Manual for detailed instructions then complete our Sample Submission Form. For Metabolomics customers, instructions to properly prepare your samples for submission can be found in our Metabolomics Sample Prep document.

If you have any questions, you can find our contact information in the "CONTACT US" tab. For pricing see the "SERVICE FEES" page.

 

Full Services Metabolomics Offering


Metabolomics services include:

  • Untargeted Quantification of a wide range of small molecules
    • Primary metabolism using GC-MS and NIST/Fiehn Libraries – GC-MS Untargeted assay - Flat Fee/sample.
    • Secondary metabolism using LC-MS (HILIC and/or RP separations) combined to Data processing using Compound Discoverer integrating several libraries (NIST Library, mzCloud, in-house library) - LC-MS Untargeted Assay - Contact us for quote.
    • Small Peptides (2-3 amino acids long) using LC-MS (HILIC and/or RP separations) combined to Data processing using Progenesis QI integrating the NIST library - LC-MS Untargeted Assay - Contact us for quote.
  • Targeted Quantification using HPLC, GCMS or LCMS. See table below for a full list of compounds and costs.
  • See "METABOLOMICS PLATFORM" tab for more information on our metabolomics instrumentation.
HPLC Targeted Assay - Flat Fee/sample
Free Amino Acids (25)Ala, Arg, Asn, Asp, Cys, Cys2, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, Val, Tau, GABA, Orn, HyPro
Hydrolyzed Amino Acids (17)Arg, Ile, Leu, Val, Phe, Tyr, Asp, Glu, MetS, CyA, Ser, Thr, Arg, His, Lys, Gly, Pro
Carotenoids/ Xantophylls (11)Neoxanthin, Zeaxanthin, Lutein, Antheraxanthin, Violaxanthin, alpha-Cryptoxanthin, beta-Cryptoxanthin, alpha-Carotene, beta-Carotene, Chlorophyll A, Chlorophyll B
GC-MS Targeted Assay - Flat Fee/sample
Free Sugars (11)Fructose, Sucrose, Glucose, Raffinose, Arabinose, Mannose, Xylose, Galactose, Lactose, Pinitol, Trehalose
Short Chain Fatty Acids (SCFAs) (9)Acetic Acid, Formic Acid, Propionic Acid, Butyric Acid, Isobutyric Acid, 2-Methylbutanoic Acid, Valeric Acid, Isovaleric Acid, Hexanoic Acid
Fatty Acid Methyl Esters (FAMEs) (14)Methyl Octanoate, Methyl Decanoate, Methyl Laurate, Methyl Myristate, Methyl Palmitate, Methyl Palmitoleate, Methyl Stearate, Methyl Oleate, Methyl Linoleate, Methyl Arachidiate, Methyl Linoleate, Methyl Behenate, Methyl Erucate, Methyl Lignocerate
LC-MS Targeted Assay – “A la carte” - Contact us for quote
Polyamines Metabolism (7)Arginine, Ornithine, Methionine, Agmatine, Putrescine, Spermine, Spermidine
Non-Mevalonate Pathway (7)1-Deoxy-D-xylulose 5-phosphate (DXP), 2-C-methylerythritol 4-phosphate (MEP), 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP), Dimethylallyl pyrophosphate (DMAPP), (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (HDMAPP), Isopentenyl pyrophosphate (IPP)
Free Amino Acids (25)Ala, Arg, Asn, Asp, Cys, Cys2, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, Val, Tau, GABA, Orn, HyPro
Bile Acids (16)Cholic Acid, Chenodeoxycholic Acid, Deoxycholic Acid, Glycocholic Acid, Glycochenodeoxycholic Acid, Glycodeoxycholic Acid, Glycolithocholic Acid, Hyocholic Acid, Lithocholic Acid, Taurocholic Acid, Taurodeoxycholic Acid, Taurochenodeoxycholic Acid, Taurolithocholic Acid, Alpha-muricholic Acid, Beta-muricholic Acid, Omega-muricholic Acid
Steroids (23)Aldosterone, Androsterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Cortexolone, Cortexone, Dehydroepiandrosterone, Dehydroepiandrosterone sulfate, 5α-Dihydrotestosterone, Estriol, Estrone, Estradiol, Etiocholanolone, 2-Methoxyestradiol, 17-Hydroxypregnenolone, Pregnenolone, allo-Pregnanolone, beta-Pregnanolone, Progesterone, 17a-Hydroxyprogesterone, Testosterone
Pyrimidine and Purine Pathway (39)   Adenine, Adenosine, AMP, ATP, Guanine, Guanosine, GMP, GDP, GTP, Deoxyadenosine, dAMP, dADP, dATP, Deoxyguanosine, dGMP, dGTP, Cytidine, Cytosine, CMP, CDP, CTP, Deoxycytosine, dCMP, dCDP, dCTP, Uracil, Uridine, UMP, UDP, UTP, Deoxyuridine, dUMP,  dUTP, dTDP, Thymine, Thymidine, dTMP/TMP, dTDP/TDP, dTTP/TTP
TCA Cycle/ Glycolysis/ Pentose Phosphate Pathway (20)Pyruvic Acid, Glutamic Acid, Aconitate, Lactic Acid, Fumaric Acid, Succinic Acid, Malic Acid, Phosphoenolpyruvate, Dihydroxyacetone phosphate, Glycerol 3-phosphate, 3-Phosphoglyceric Acid, 2-Phosphoglyceric Acid, Citric Acid, Ribose-5-phosphate, Fructose-6-Phosphate, Glucose-6-Phosphate, Glucose-1-Phosphate, Fructose-1,6-Bisphosphate, Sedoheptulose-7-Phosphate, Erythrose-4-Phosphate

 

 

 

Phytohormones (43)

“Common” Panel

Abscisic acid (ABA), Abscisic Acid Glucose Ester (ABA-GE), Salicylic acid (SA), Jasmonic acid (JA), JA-Ile, JA-Leu, Methyl-JA, 12-oxo-phytodienoic acid (OPDA), Indole-3-acetic acid (IAA), Methyl-IAA, IAA-Asp, IAA-Ala, IAA-Trp, IAA-Glu, 6-Benzylaminopurine, Indole-3-butyric acid (IBA), Indole-3-carboxylic acid, Indole-3-lactic acid, 3-Indolepropionic acid, N6-2-isopentanyladenine, trans-Zeatin, cis-Zeatin, Dihydrozeatin (DHZ), trans-ZRiboside, cis-ZRiboside

“Expanded” Panel (A la carte):

Strigol, 5-Deoxystrigol, Orobanchol DIMBOA, GA1, GA3, GA4, GA8, GA9, GA12, GA19, GA20, GA24, GA29, GA53, Brassinolide, Benzoic acid, Kinetin

 

 

 

 

 

Flavonoids, Phenolic Acids, and more (67)

“Common” Panel: 

Anthocyanins and Proanthocyanidins: Delphinidin, Proanthocyanidin A2, Procyanidin B1;  

Hydroxybenzoic and Hydroxycinnamic acids : Protocatechuic Acid, Vanillic Acid, Gallic Acid, Ellagic acid, Syringic Acid, Caffeic Acid, Cinnamic Acid, Chlorogenic acid, Coumaric Acid, Ferulic Acid, Sinapic acid; 

Flavones and Isoflavones: Apigenin, Luteolin, Daidzein, Genistein;

Flavonols and Flavonones: Catechin, Epicatechin, Epigallocatechin, Epicatechin Gallate, Kaempferol, Quercetin, Hesperetin, Naringenin; 

Flavonol glycosides and Flavanone glycosides: Quercetin-3-Glucoside, Quercetin-3-Galactoside, Rutin; Chalcones and others: Phloretin, Chalconaringenin, Resveratrol

“Expanded” Panel (A la carte}

Anthocyanins and Proanthocyanidins: Cyanidin-3-glucoside, Cyanidin-3-rutinoside, Apigeninidin, Procyanidin B2, Procyanidin B3, Procyanidin B4, Procyanidin C1, Procyanidin C2

Hydroxybenzoic and Hydroxycinnamic acids: Hydroxybenzoic acid, 4-Methoxycinnamic acid, Cryptochlorogenic acid

Flavones and Isoflavones: Chrysin, Glycitein, Biochanin A, Formononetin. Tricin

Flavonols and Flavonones: Isorhamnetin, Fisetin, Myricetin, Galangin, Pinocembrin, Pinobanksin

Flavonol glycosides and Flavanone glycosides: Kaempferol 3-O- β-rutinoside, Naringin, Diosmin, Herperidin, Swertisin

Chalcones and others: Coumestrol, Coumarin,  Caffeine, Trigonelline, Matairesinol, Secoisolariciresinol, Lariciresinol, Mangiferin, Mimosine 

Alpha and Beta Acids (6)Cohumulone, Humulone, Adhumulone, Colupulone, Lupulone, Adlupulone