Proteomics Platform

Proteomics Platform

• Proteomics of whole organisms, organs, organelles, tissues, cell pellets, pulldowns, complex or single protein mixes from solutions or gels
• Quantification by label-free or multiplexed labeling (e.g., TMT-10/16plex) techniques with microscale sub-fractionation to increase coverage
• High resolution/high mass accuracy data for intact mass protein analysis
• Phosphoproteomics using TiO2 phosphopeptide enrichment
• Technological platforms supported by bioinformatics tools to process data and report results

• Post-translational modification characterization

   

Thermo Orbitrap Eclipse Tribrid with FAIMS and ETD coupled to a Dionex U3000 RSLCnano

Resolving power up to 500,000 FWHM MS acquisition rate up to 40 Hz Flow rate of the nanopump: 20 nL/min to 1.5 µL/min Pressure to 800 bar (11,600 psi)

Thermo Q-Exactive – High Field (QE-HF) coupled to Thermo Vanquish H binary UPLC

High Resolution/Accurate Mass (HR/AM) instrument Resolving power up to 240,000 at 200 m/z Sub ppm mass accuracy Scan speed of 18 Hz at 15,000 resolution Flow rate of the nanopump: 20 nL/min to 1.5 µL/min Pressure to 800 bar (11,600 psi)

❖ Mascot 2.6.1 (Matrix Science Ltd) for protein identification from MS/MS spectra

❖ Proteome Discoverer 2.4 Search Engine (Thermo) for metabolic, isotope labeling and label free quantitative proteomics

❖ Scaffold 4.8.9 (Proteome Software) for protein identification, reporting and label-free quantitative proteomics

❖ PEAKS 8 (Bioinformatics Solutions Inc) for de novo sequencing and PTM characterization