Full Services User Overview
A virtual consultation with the PMF Director is required prior to submitting samples to check for compatibility of sample type and format with our analytical approaches and to confirm the experimental approach and expectations for the services. Please review the Full Service Users section of our Policies and Procedures Manual for detailed instructions then complete our Sample Submission Form. For Metabolomics customers, instructions to properly prepare your samples for submission can be found in our Metabolomics Sample Prep document.
If you have any questions, you can find our contact information in the "CONTACT US" tab. For pricing see the "SERVICE FEES" page.
Full Services Offering
Proteomics services include:
- Bottom-up Proteomics of whole organisms, organs, organelles, tissues, cell pellets, pulldowns, complex or single protein mixes from solutions or gels.
- Quantification by label-free or multiplexed labeling techniques (eg TMT-10/16plex) with microscale subfractionation to increase coverage.
- Post Translational Modification Characterization especially phosphopeptide enrichment from whole organism digests, but also ubiquitylation, acetylation, etc.
- See "PROTEOMICS PLATFORM" tab for more information on our proteomics instrumentation.
Metabolomics services include:
- Untargeted Quantification of a wide range of small molecules
- Primary metabolism using GC-MS and NIST/Fiehn Libraries
- Secondary metabolism using LC-MS and Progenesis QI, NIST Library, mzCloud
- Targeted Quantification using HPLC or LCMS. See table below for a full list of compounds.
- See "METABOLOMICS PLATFORM" tab for more information on our metabolomics instrumentation.
| Targeted Primary Metabolites | |
| Polyamines | Putrescine, Spermine, Spermidine |
| Pyrimidine Pathway | ATP, GTP, dATP, dGTP, dCTP, UTP, dUTP, Uridine, UMP, UDP, dCDP, CTP, CDP, Cytidine, dUMP, Deoxyuridine, dTDP, Thymidine, dTTP, car-Asp, dTMP |
| Free Sugars | Fructose, Sucrose, Glucose, Raffinose, Arabinose, Mannose, Xylose, Galactose, Lactose, Pinitol, Trehalose |
| Free Amino Acids | Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, Val, Tau, GABA, Orn, HyPro |
| Hydrolyzed Amino Acids | Arg, Ile, Leu, Val, Phe, Tyr, Asp, Glu, MetS, CyA, Ser, Thr, Arg, His, Lys, Gly, Pro |
| TCA Cycle/ Glycolysis/ Pentose Phosphate Pathway | Pyruvic Acid, Glutamic Acid, Aconitate, Keto-Glutaric Acid, Lactic Acid, Fumaric Acid, Succinic Acid, Malic Acid, Phosphoenolpyruvate, Glyceraldehyde 3-phosphate, Dihydroxyacetone phosphate, L-Glycerol 3-phosphate, D-(-)-3-Phosphoglyceric Acid, D-(+)-2-Phosphoglyceric Acid, Citric Acid, Ribose-5-phosphate, Fructose-6-Phosphate, Glucose-6-Phosphate, Glucose-1-Phosphate, Fructose-1,6-Bisphosphate, Sedoheptulose-7-Phosphate, Succinyl Coenzyme A, Erythrose-4-Phosphate, Ribulose-5-Phosphate |
| Short Chain Fatty Acids | Acetic Acid, Formic Acid, Propionic Acid, Butyric Acid, Isobutyric Acid, 2-Methylbutanoic Acid, Valeric Acid, Isovaleric Acid, Hexanoic Acid |
| Fatty Acid Methyl Esters (FAMEs) | Methyl Octanoate, Methyl Decanoate, Methyl Laurate, Methyl Myristate, Methyl Palmitate, Methyl Palmitoleate, Methyl Stearate, Methyl Oleate, Methyl Linoleate, Methyl Arachidiate, Methyl Linoleate, Methyl Behenate, Methyl Erucate, Methyl Lignocerate |
| Targeted Secondary Metabolites | |
| Non-Mevalonate Pathway | DXP, MEP, CDP-ME, MEcPP, DMAPP, HDMAPP, IPP |
| Phytohormones (inclu. Gibberellins) | ABA, SA, JA, JA-Ile, OPDA, IAA, IAA-Asp, Methyl-IAA, IAA-Ala, IAA-Trp, t-Zeatin, c-Zeatin, t-ZRiboside, c-ZRiboside, Strigol, 5-Deoxystrigol, Orobanchol, DIMBOA, GA1, GA3, GA4, GA8, GA9, GA12, GA19, GA20, GA24, GA29, GA53 |
| Flavonoids and Phenolic Acids | Acacetin, Apigenin, Catechin, Chlorogenic Acid, Delphinidin, Daidzein, Epicatechin, Genistein, Hesperetin, Kaempferol, Luteolin, Naringenin, Phloretin, Proanthocyanidin A2, Procyanidin B2, Protocatechuic Acid, Quercetin-3-Glucoside, Quercetin-3-Galactoside, Chalconaringenin, Quercetin, Rutin, Syringic Acid, Vanillic Acid, Resveratrol, Caffeic Acid, Cinnamic Acid, p-Courmaric Acid, Ferulic Acid, Gallic Acid |
| Carotenoids/ Xantophylls | Zeaxanthin, Lutein, Antheraxanthin, Violaxanthin, Chlorophyll A, Chlorophyll B |
| Bile Acids | Cholic Acid, Chenodeoxycholic Acid, Deoxycholic Acid, Glycocholic Acid, Glycochenodeoxycholic Acid, Glycodeoxycholic Acid, Glycolithocholic Acid, Hyocholic Acid, Lithocholic Acid, Taurocholic Acid, Taurodeoxycholic Acid, Taurochenodeoxycholic Acid, Taurolithocholic Acid, Alpha-muricholic Acid, Beta-muricholic Acid, Omega-muricholic Acid |
| Alpha and Beta Acids | Cohumulone, Humulone, Adhumulone, Colupulone, Lupulone, Adlupulone |