Useful Links

NanoAnalyzer Publication List – A rapidly growing list of publications that have utilized the NanoFCM NanoAnalyzer System.

Purdue Cytometry Discussion List – Ongoing discussion platform related to a multitude of flow cytometry topics.

Fluorofinder – Experiment Design & Reagent Search Platform – After selecting “Build a Panel,” search for “University of Nebraska-Lincoln (UNL)” in order to use settings specific to the Service Center’s instruments.

Thermofisher Panel Builder - Tool designed by Thermofisher for designing multi-parameter flow cytometry panels.

The Daily Dongle – Archive of FlowJo related webinars and discussions. 

FlowJo YouTube Channel – Indepth video tutorials covering a wide range of FlowJo topics.

Flow Cytometry – A Basic Introduction by DeNovo Software – A useful introductory book discussing the basics of flow cytometry.

Optimized Multicolor Immunofluorescence Panels (OMIPs) – Peer-reviewed articles describing optimized multicolor panels. These are great resources to utilize when building new panels, as they show what antibodies and fluorochromes work well together for specific cell types.

Practical Flow Cytometry – By Howard Shapiro – This free download of the 4th edition of this text offers very detailed discussions related to hardware, software, and applications.


Fluorescence Spectrum Viewers

BD Fluoroscence Spectrum Viewer

Thermofisher Fluorescence Spectra Viewer

BioLegend Fluorescence Spectra Analyzer


Boilerplate Text

The Flow Cytometry Core Facility is a part of the Center for Biotechnology at the University of Nebraska–Lincoln. The Center provides users with instrumentation, support, and technical services for cell sorting and analysis. The Core currently manages and maintains two analysis instruments for use, including a 4-laser/16 color Beckman Coulter CytoFLEX LX, and a 2-laser/ 2 color NanoFCM NanoAnalyzer designed to measure nanoscale particles. The cell sorter is a 4-laser/15-color Beckman Coulter CytoFLEX SRT, and it is housed in a Baker BioProtect biosafety cabinet making it possible to sort BSL2 samples. The SRT is capable of sorting up to four populations at once and is equipped with a versatile collection device which can be used for sorting in multi-well plates or slides. The Core's Director, Dirk Anderson nearly 20 years of flow cytometry experience, in both academia and industry. Along with analysis and sorting, the Core also offers training, protocol development assistance, and data analysis.