CryoEM Workshop

May 2, 2024

Promotional flyer for a CryoEM Workshop on biomolecule analysis using conventional and cryo‑electron microscopy. Lecture and demo sessions are May 20–21 with coffee breaks and lunch included. An optional hands‑on workshop runs May 22–24 for an additional fee and limited participants. The flyer includes a QR code to claim a spot, workshop sponsor information, and logos for Thermo Fisher Scientific, the University of Nebraska–Lincoln, and the Nebraska Center for Biotechnology.

CryoEM Workshop
Lectures/Demonstration May 20 & 21, 2024
Hands-On Training May 22, 23, or 24

Registration is now closed
 

$100.00 per person for lecture/demo. $50.00 additional for hands-on training day.
NCIBC is sponsoring the first ~6 CIBC students for the hands-on training.

Deadline to register is May 15th or until full.

 

This two-day workshop on CryoEM and Negative Stain is designed for Graduate Students, Postdocs, and Faculty who want to learn the basics of room-temperature electron microscopy and Cryo-Electron microscopy. CryoEM is a rapidly growing technique used in structural biology, small molecule, and protein crystallography. Additionally, an optional daylong hands-on experience will be offered for ten participants on a first-come, first-served basis to learn both negative stain and CryoEM methods.

Workshop is sponsored by: The Nebraska Center for Integrated Biomolecular Communication (NCIBC), Thermo Fisher, and The Nebraska Center for Biotechnology

 

The Workshop will include (Limited to 15 attendants):

  • Room temperature Microscopy Techniques.
  • Image formation in electron microscopy
  • Sample preparation in CryoEM.
  • CryoEM Workflows
  • Capabilities of the UNL 200kV Glacios CryoTEM
  • Single particle CryoEM processing tutorial.
  • Sample preparation Tutorial and imaging.

The Hands-on part will include (Limited to 10 participants):

NCIBC is sponsoring (FREE) the first ~6 CIBC students for the hands-on training.
During registration, mark that you are a CIBC student.

  • Sample preparation for both Negative stain and CryoEM.
  • Gid Clipping (CryoEM)
  • Sample Loading.
  • Sample imaging.
 

 

Preliminary Schedules

May 20th - Lecture Day, Beadle Center E106

9:00 am - Arrival and pick-up of registration badge
9:15 – 10 am - Fundamentals of Electron Microscopy
10:00- 10:15 am - Break
10:15 am- 11 am - Negative stain sample preparation
11:00- 11:45 am – Image Formation and processing in Electron Microscopy
11:45 am -1:00 pm – Lunch Break, Provided by Thermo Fisher 
1:00 - 1:45 pm - CryoEM Sample preparation and workflows
1:45 –2:00 pm – Break
2:00 – 2:45 – 200kV Glacios CryoTEM
2:45-3:00 pm Break
3:00-4:00 pm CryoEM data Processing tutorial
4:00-4:45 pm Q&A

May 21st - Demo Day Beadle & Morrison Centers

9:00 am- 12:00 pm - Group 1 (Negative stain), Group 2 (CryoEM)
12:15 pm - 1:15 pm - Lunch Break, Provided by CIBC 
1:30 – 4:30 pm - Group 2 (Negative stain), Group 1 (CryoEM)

 

May 22nd - 24th - Optional Training Day

~9:00 am- 5:00 pm - Lunch on own 

Participants can bring their own sample to run.
The sample should be a Protein/RNA/DNA with a concentration of at least 1 mg/mL and a size of about 100kDa is required. Will need ~20uL.

 


Each participant will elect for a day during registration. Selection will be first come first serve.

 

Please contact CryoEM Director, Eduardo Romero Camacho (eduardo.romero@unl.edu) or Admin Associate, Kaitlin Bachle (kbachle2@unl.edu) if you have questions or concerns.

 

 


 

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