CryoEM Workshop

May 2, 2024

CryoEM May 2024 Workshop Flyer Information

CryoEM Workshop
Lectures/Demonstration May 20 & 21, 2024
Hands-On Training May 22, 23, or 24

Registration is now closed
 

$100.00 per person for lecture/demo. $50.00 additional for hands-on training day.
NCIBC is sponsoring the first ~6 CIBC students for the hands-on training.

Deadline to register is May 15th or until full.

 

This two-day workshop on CryoEM and Negative Stain is designed for Graduate Students, Postdocs, and Faculty who want to learn the basics of room-temperature electron microscopy and Cryo-Electron microscopy. CryoEM is a rapidly growing technique used in structural biology, small molecule, and protein crystallography. Additionally, an optional daylong hands-on experience will be offered for ten participants on a first-come, first-served basis to learn both negative stain and CryoEM methods.

Workshop is sponsored by: The Nebraska Center for Integrated Biomolecular Communication (NCIBC), Thermo Fisher, and The Nebraska Center for Biotechnology

 

The Workshop will include (Limited to 15 attendants):

  • Room temperature Microscopy Techniques.
  • Image formation in electron microscopy
  • Sample preparation in CryoEM.
  • CryoEM Workflows
  • Capabilities of the UNL 200kV Glacios CryoTEM
  • Single particle CryoEM processing tutorial.
  • Sample preparation Tutorial and imaging.

The Hands-on part will include (Limited to 10 participants):

NCIBC is sponsoring (FREE) the first ~6 CIBC students for the hands-on training.
During registration, mark that you are a CIBC student.

  • Sample preparation for both Negative stain and CryoEM.
  • Gid Clipping (CryoEM)
  • Sample Loading.
  • Sample imaging.
 

 

Preliminary Schedules

May 20th - Lecture Day, Beadle Center E106

9:00 am - Arrival and pick-up of registration badge
9:15 – 10 am - Fundamentals of Electron Microscopy
10:00- 10:15 am - Break
10:15 am- 11 am - Negative stain sample preparation
11:00- 11:45 am – Image Formation and processing in Electron Microscopy
11:45 am -1:00 pm – Lunch Break, Provided by Thermo Fisher 
1:00 - 1:45 pm - CryoEM Sample preparation and workflows
1:45 –2:00 pm – Break
2:00 – 2:45 – 200kV Glacios CryoTEM
2:45-3:00 pm Break
3:00-4:00 pm CryoEM data Processing tutorial
4:00-4:45 pm Q&A

May 21st - Demo Day Beadle & Morrison Centers

9:00 am- 12:00 pm - Group 1 (Negative stain), Group 2 (CryoEM)
12:15 pm - 1:15 pm - Lunch Break, Provided by CIBC 
1:30 – 4:30 pm - Group 2 (Negative stain), Group 1 (CryoEM)

 

May 22nd - 24th - Optional Training Day

~9:00 am- 5:00 pm - Lunch on own 

Participants can bring their own sample to run.
The sample should be a Protein/RNA/DNA with a concentration of at least 1 mg/mL and a size of about 100kDa is required. Will need ~20uL.

 


Each participant will elect for a day during registration. Selection will be first come first serve.

 

Please contact CryoEM Director, Eduardo Romero Camacho (eduardo.romero@unl.edu) or Admin Associate, Kaitlin Bachle (kbachle2@unl.edu) if you have questions or concerns.

 

 


 

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