July 15, 16, and 17, 2019 Summer Biotechnology Workshops
Registration deadline is July 1, 2019.
This two-half day introductory Proteomics Workshop is intended to educate students and faculty about some of the technologies and methodologies in the field of mass spectrometry-based proteomics. The workshop will cover the topics:
- Hands-on workshop about protein identification using a bottom-up approach.
- Sample preparation for liquid chromatography and mass spectrometry.
- Interactive data analysis session.
The two half day sessions will focus on confocal microscopy, flow cytometry and electron microscopy. The workshop will cover the topics:
- Real-time and time lapse image analysis using live plant or animals cells, or microbes.
- Quantitative fluorescence analysis using FACS-sorting and confocal images (or other specific imaging methods of interest to participants).
- Transmission electron microscopy using negative staining methods and ultrastructural analysis.
- Topographic analysis by scanning electron microscopy (detection of secondary or back-scattered electron signals).
The main focus of this workshop will be on the analysis of microbiome data obtained via amplicon sequencing, with special emphasis on the efficient handling of hundreds of samples on high performance computing clusters. The workshop will cover the topics:
- Use of R in a Linux environment (command-line) or in Jupyter notebooks.
- Gain familiarity with the Linux environment, complete a case study from raw DNA sequence to a fully annotated small microbial genome. We follow this with short primer on the basics of the R language to get everyone onboard.
- 16S taxonomic profiling of a microbiome, using R and Bioconductor packages, from quality control to data visualization.