Proteomics and Metabolomics Workshop
October 11 & 18, 2023
Registartion is now Closed
$50.00 per person, per session. Space is limited to 15 participants per day.
October 11 - Metabolomics
This one-day Metabolomics Workshop is intended to introduce students and faculty to targeted and untargeted metabolomics, a rapidly growing technology in clinical diagnostics, drug discovery, food science, agriculture and environmental sciences; and broaden participants’ understanding of the data processing and insight differences between untargeted and targeted assays.
The workshop will include:
- Hands-on experience with sample preparation.
- Method optimization for targeted assay.
- Interactive data analysis session.
Limited to 15 attendees.
3 DEMOS sessions (1h each*)
- Metabolite extraction (liquid-liquid extraction, tissue lyzer)
- Amino acid derivatization and UPLC (AccQ-Tag derivatization, UPLC set up)
- QTRAP direct infusion (standard instrument optimization – Low resolution, targeted metabolomics)
*Groups of 5 will be rotating through the sessions throughout the day
October 18 - Proteomics
This one-day Proteomics Workshop is intended to educate students and faculty about some of the technologies and methodologies in the field of mass spectrometry-based proteomics. Our hands-on workshop will cover both practical and theoretical aspects of performing protein identification using a bottom-up approach. We will discuss sample preparation to liquid chromatography and mass spectrometry methods, through laboratory session and interactive data analysis session.
Limited to 15 attendees.
3 DEMOS sessions (1h each*)
- SP3 Protein cleanup (contaminant removal, one-pot sample digestion)
- Peptides zip tipping/QE direct infusion (mass spectrometry data acquisition of peptides)
- LC-MS/MS on the Eclipse (high resolution mass spectrometry – peptides separation)
*Groups of 5 will be rotating through the sessions throughout the day
Preliminary Schedule:
8:45 am – Arrival and pick-up registration badge
9:00 – 9:45 am – Introduction
9:45 – 10:00am - Break
10:00 – 11:00 am – Demo Session 1
11:00 – 11:15 am – Break
11:15 – 12:15 am – Demo Session 2
12:15 am – 1:30 pm Lunch break
1:30 – 2:30 pm – Demo Session 3
2:30 – 2:45 pm – Break
2:45 – 3:45 pm – Data analysis
3:45 – 4:00 pm – Break
4:00 – 4:45 pm – Question and Answer
Please contact Sophie Alvarez (salvarez@unl.edu) or Kaitlin Bachle (kbachle2@unl.edu) if you have questions or need to cancel your registration.