CryoEM Features

Scroll through the CryoEM #BiotechMonday Features 

Informational graphic describing the PELCO easiGlow Glow Discharge Cleaning System. The image shows the easiGlow unit on a laboratory bench with a clear cylindrical glass chamber, digital control panel, and a connected vacuum pump via metal tubing. Text explains that the system cleans and hydrophilizes TEM carbon films using glow discharge, making it essential for preparing Cryo and negative stain electron microscopy grids. Decorative red, navy, and gray geometric shapes appear on the right side.

 

 

 

 

 

 

Informational graphic about apoferritin used as a benchmark sample in Cryo‑electron microscopy. The center shows a detailed gray structural model of the apoferritin protein complex. Text explains that apoferritin is a highly symmetrical protein composed of 24 subunits, approximately 440 kDa in mass, involved in iron regulation, and has achieved a world‑record CryoEM resolution of 1.20 Å. Decorative diamond patterns frame image.

 

 

Educational graphic explaining the first step in CryoEM using negative stain electron microscopy. Top text reads “Did you know?” and describes staining samples with a uranium salt to enhance contrast and evaluate shape, homogeneity, orientation, and distribution. Below are two images: a grayscale apoferritin negative‑stain micrograph with a 500 nm scale bar, and a simple diagram illustrating negative‑stain sample preparation on a thin carbon film showing specimen and stain placement.

 

 

Informational graphic showing a Thermo Fisher Scientific 200kV Glacios CryoTEM installed in a biotechnology facility. The instrument is shown in a hallway‐style lab space with control cabinets and a screen on the front. Accompanying text explains that the CryoEM Core’s flagship instrument includes a Selectris energy filter, CetaD camera, and Falcon 4i camera, enabling high‑throughput single‑particle CryoEM imaging. University of Nebraska and Nebraska Center for Biotechnology logos appear at the bottom..

 

 

Laboratory image showing a TFS Vitrobot Mark IV used for cryogenic preservation of samples. The instrument is shown freezing a sample in liquid ethane inside a chilled metal chamber marked with a cold‑hazard symbol. Overlaid text explains that rapid freezing prevents ice crystal formation by solidifying water as vitreous ice, preserving sample structure for imaging.