Microscopy

MICROSCOPY
MICROSCOPY

Services

Morrison Microscopy Core Research Facility

Located on the first floor of the George W. Beadle building, the Morrison Microscopy Core Research Facility has state of the art imaging systems including light/fluorescence microscopes, confocal laser scanning microscopes and electron microscopes. The microscopy core facility will provide strong technical support for multi-disciplinary research in the state of Nebraska. We will also provide services, teaching and training for university research communities.

Supported by: 1) Nebraska State funding by the Nebraska Research Initiative; 2) Federal funding by NIH COBREs (NCV and Redox); 3) A private donation from the Ken Morrison Family; 4) NU Foundation; and 5) usage/service fees.

New user to this core?

If you are new to the Microscopy core and need to use one of the image tools of the core, please fill out the Request for Microscopy Services form and send it with your email to Dr. Y. Joe Zhou or one of other microscopy specialists of the core (See Personnel tab). In order for us to assist you efficiently, we need to have some background information about your research project(s) related to microscopy so that we can identify which imaging system is the best fit for your experiment and what kind of training is needed.Request for Microscopy Services Form

We will follow up your request and schedule an appointment with a core specialist to discuss some specifics on sample preparation and imaging collections in the Microscopy core.

Service Fees

Note: Up to 4 hrs at assisted rate will be applied to all new users (training fee included).
Click here for printable copy of fees.

Request for Microscopy Services Form

ServiceAcademic/In HouseIndustry/Private Company Users
Confocal $35/hour $100/hour (Assisted)
Fluorescence/digital $15/hour $50/hour (Assisted)
TEM/SEM - Assisted $50/hour $150/hour (Assisted)
TEM - Unassisted $35/hour $100/hour
Variable-pressure SEM $30/hour $100/hour
Field-emission SEM $30/hour $100/hour
Laser Capture Micro-dissection System $15/hour plus the cost for disposable materials $40/hour plus the cost for disposable materials
SEM Sample Preparation $20/sample $60/sample
TEM Negative Stain $26/sample ($10 additional grid) $70/sample ($20 additional grid)
Standard TEM Preparation (fix, embed, section and stain) $53/sample (1 block, 2 grids) ($10 for each additional TEM grid, $40 for two grids from each additional block) $120/ first sample ($30 for each additional TEM grid)
Additional sectioning/straining of the embedded samples $27/sample $60/sample
Frozen and paraffin Sectioning by user $30/sample plus material cost
$30/use (up to 4 hrs)
$30 training
$60/sample (Assisted)
$60/use
Sputter Coating $20/run (Free when examining the samples using this core’s SEMs) $55/run

Instruments & Facilities

Advanced Fluorescence Microscopes

Confocal Microscopes

Olympus FV500 Inverted (Olympus IX-81) Confocal Microscope

The inverted (Olympus IX-81) confocal microscopes use confocal optics for high resolution, high contrast and increased resolution in the light axis direction. Acquisition functions include 3D construction, Z series sectioning, time series observations, sequential laser scan and image analysis functions. A total of 5 channels can be acquired simultaneously with 4 fluorescence images and a transmitted light image. The confocal has 6 laser lines (405, 458, 488, 514, 543, and 633 nm) and can be used for multiple fluorescence labeling detection.
Microscope
Olympus FV500 Inverted (Olympus IX-81) Confocal Microscope


Nikon A1 confocal system

The Nikon A1 confocal system on a Nikon 90i upright fluorescence microscope can provide 6 different excitation laser lines, 405, 457, 476, 488, 514, 561, and 641nm. It is a standard four-channel confocal imaging system but also has a spectral detector allowing for 10 nm, 6 nm and 2.5 nm resolution of emission spectra across up to 32 channels.
Microscope
Nikon A1 confocal system


Inverted Fluorescence Microscope

Nikon Ti-S Inverted Fluorescence Microscope with Digital camera allows researchers to collect images under sterile conditions.
 
Inverted Fluorescence Microscope


Laser Capture Microdissection System

Laser Capture Microdissection system (LCM, PixCell IIe from Arcturus) provides one of the best tools available to date for isolation of individual cells or specific populations of cells, or bacteria of interest from tissue sections for further molecular and biochemical analyses.
Microscope
Laser Capture Microdissection System


Upright Fluorescence Microscope Olympus AX70

Upright fluorescence microscope (Olympus AX70) with digital camera.
Microscope
Upright Fluorescence Microscope Olympus AX70


Stereo Fluorescence Microscope Nikon SMZ800

Stereo fluorescence microscope (Nikon SMZ800) with digital camera that allows users to examine samples under sterile conditions for phenotypic selections.
 Microscope
Stereo Fluorescence Microscope Nikon SMZ800


EVOS® FL Auto Cell Imaging System

EVOS® FL Auto Cell Imaging System with an automated microscopic stage (computer controlled X-Y-Z movement), dual cameras, selectable excitation/emission filters from 5 LED Light Cubes (DAPI, GFP, RFP, Texas Red, and Cy5), and 3x optical zoom over different lenses (4x, 10x, 20x, 40x, 60x, and 100x). This system is user friendly and suitable for single or multi-labeling fluorescence or colorimetric image acquisitions from sections or live cells using single culture dish, 6, 12, or 24-well culture plates, or 96 well ELISA plate.
EVOS® FL Auto Cell Imaging System


Electron Microscopes

Transmission Electron Microscope

Hitachi H7500 TEM is an advanced electron microscope with a window-based computerized operating system for a) ultrastructural analysis on ultrathin sections of samples, b) assay of nanoparticles, and c) examination of negative stained microbial particles (such as virus) and nanoparticles. A bottom-mount high-resolution CCD camera is installed with this system.

Magnification range: 50x to 200,000x (HC mode at 80 KV); Resolution (point to point): up to 0.5 nm
Microscope
Transmission Electron Microscope


Field-Emission Scanning Electron Microscope

Hitachi S4700 Field-Emission SEM is a powerful tool for topographic analysis at nano-scale levels, which uses windows-based computerized operating system with the high-resolution digital processing capacity.

Magnification range: 20x to 500,000x; Resolution: up to 1.0 nm
Microscope
Field-Emission Scanning Electron Microscope


Variable Pressure Scanning Electron Microscope

Hitachi S-3000N Variable-Pressure SEM is an advanced electron microscopic imaging system with the capability of using high vacuum or a variable pressure range from 1-270 Pa. This SEM uses a Windows -based computerized operating system with high-resolution digital processing capacity and is most suitable for topographic analysis using biological or geological samples.

Magnification range: 25x to 200,000x; Resolution: up to 3 nm in high vacuum mode and up to 5 nm in variable pressure mode
Microscope
Variable Pressure Scanning Electron Microscope

Personnel

Research/Service Group

Christian Elowsky
BEAD E119.5
Phone: 402-472-5942
Email Christian

Terri Fangman
E119.5 J BEAD
Phone: 402-472-5942
Email Terri

Publications

2011-

Virdi, K., Wamboldt, Y., Kundariya, H., Laurie, J., Keren, I., Kumar, K.R.S, Block, A., Bassett, G., Luebker, S., Elowsky, C., Day, P., Roose, J., Bricker, T., Elthon, T., Mackenzie, S.  2016.  MSH1 is a plant organellar DNA binding and thylakoid protein under precise spatial regulation to alter development.  Molecular Plant 9(2) 245-260.

Guo, M., Kim, P., Li, G., Elowsky, C., Alfano, J.  2016. A bacterial effector co-opts calmodulin to target the plant microtubule network.  Cell Host & Microbe 19, 67-78.

Navarro-Yepes, J., Anandhan, A., Bradley, E., Bohovych, I., Yarabe, B., de Jong, A., Ovaa, H., Zhou, Y., Khalimonchuk, O., Quintanilla-Vega, B., Franco Cruz, R. 2015. Inhibition of Protein Ubiquitination by Paraquat and 1-Methyl-4-Phenylpyridinium Impairs Ubiquitin-Dependent Protein Degradation Pathways. Mol Neurobiol.  2015 Sept 26:1-23. DOI 10.1007/s12035-015-9414-9

Mandal, S. Y Zhou, A. Shibata, and C. J. Destache. 2015 Confocal fluorescence microscopy: An ultra-sensitive tool used to evaluate intracellular antiretroviral nano-drug delivery in HeLa cells AIP ADVANCES 5, 084803 (2015): dx.doi.org/10.1063/1.4926584

Petro, T. M., Agarkova, I., Zhou, Y., Yolken, R. H., Van Etten, J. L., Dunigan, D. 2015. Response of Mammalian Macrophages to Challenge with the Chlorovirus Acanthocystis turfacea Chlorella Virus 1. Journal of virology, 89(23), 12096-107.

Lee, D., M. M. Rahman, Y. Zhou, S. Ryu.  2015 Confocal Microscopy Indentation Method for Hydrogel. Langmuir. 2015. 31(35), 9684-93.

McAtee CO, Berkebile AR, Elowsky C, Fangman T, Barycki JJ, Wahl JK, Khalimonchuk O, Naslavsky N, Caplan S, Simpson MA.  Hyaluronidase Hyal1 Increase Tumor Cell Proliferation and Motility Through Accelerated Vesicle Trafficking. J. Biol. Chem. 2015 Apr 8. Pii: jbc.M115.647.446.

Date, A.A., A. Shibata, E. McMullen, K. L. Bruzzo, P. Bruck, M. Belshan, Y. Zhou, C. J.  Destache.  2015. Thermosensitive gel containing cellulose acetate phthalate-efavirenz combination nanoparticles for prevention of HIV-1 infection. J. Biomed. Nanotechnol. 11 (3), March 2015: 416-427.

Friesen K, Chen H, Zhu J, Taylor DB. External Morphology of Stable Fly (Diptera: Muscidae) Larvae. J Med Entomol. 2015 Jul;52(4):626-37.

Hu, M., T. Zhang, J. Stansbury, Y. Zhou, H. Chen, J. Neal. 2015. Contributions of Internal and External Fouling to Trans-membrane Pressure in MBRs: Experiments and Modeling. J. Environ. Eng.  2015 141(6): 04014097.  EE.1943-7870.0000925.

Sanford B, Li Y, Maly CJ, Madson CJ, Chen H, Zhou Y, Belshan M. 2014. Deletions in the fifth alpha helix of HIV-1 matrix block virus release. Virol.  2014 Sep 10; 468-470C:293-302. 

Block, A., Widhalm, J., Fatihi, A., Cahoon, R., Wamboldt, Y., Elowsky, C., Mackenzie, S., Cahoon, E., Chapple, C., Dudareva, N., Basset, G.  2014.  The Origin and Biosynthesis of the Benzenoid Moiety of Ubiquinone (Coenzyme Q) in Arabidopsis.  The Plant Cell 26(5) 1938–1948.

Edagwa BJ, Guo D, Puligujja P, Chen H, McMillan J, Liu X, Gendelman HE, Narayanasamy P. Long-acting antituberculous therapeutic nanoparticles target macrophage endosomes. FASEB J. 2014 Dec;28(12):5071-82.

Massilamany, C., Gangaplara A, Jia T, Elowsky C, Li Q, Zhou Y, and Reddy J. 2014. In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers: J. Vis. Exp. 2014 Aug 1; (90). doi: 10.3791/51679.

Wang, L., G. Kang, P. Kumar, W. Lu, Y. Li, Y. Zhou, Q. Li and C. Wood. 2014. Humanized-BLT Mouse Model of Kaposi's Sarcoma-Associated Herpesvirus Infection.  Proc Natl Acad Sci U S A. 2014 Feb 25; 111(8):3146-51.

Li, G., Froehlich, J., Elowsky, C., Msanne, J., Ostosh, A., Zhang, C., Awada, T., Alfano, J.  2014.  Distinct Pseudomonas type-III effectors use a cleavable transit peptide to target chloroplasts.  The Plant Journal 77 (2) 310–321.

Zhou, Y.  Confocal imaging of nerve cells; Chapter 18, in “Current Laboratory Methods in Neuroscience Research” (H. Xiong and H. Gendelman, Editors).  Springer Science + Business Media, New York. 2014, page: 235-247

Massilamany, C., Gangaplara A, Jia T, Elowsky C, Kang G, Li Q, Zhou Y, and Reddy J. 2014. Direct staining with major histocompatibility complex class II dextramers permits detection of antigen-specific, autoreactive CD4 T cells in situ. PLoS One. 2014 Jan 27; 9(1):e87519. doi: 10.1371/journal.pone.0087519.

Frese SA, Mackenzie DA, Peterson DA, Schmaltz R, Fangman T, Zhou Y, Zhang C, Benson AK, Cody LA, Mulholland F, Juge N, Walter J. 2013  Molecular Characterization of Host-Specific Biofilm Formation in a Vertebrate Gut Symbiont. PLoS Genet. 2013 Dec 9(12): e1004057 (1-13).

Guo X, Yuan L, Chen H, Sato SJ, Clemente TE, Holding DR. Nonredundant function of zeins and their correct stoichiometric ratio drive protein body formation in maize endosperm. Plant Physiol. 2013 Jul;162(3):1359-69.

Block, A., Toruno, T., Elowsky, C., Zhang, C., Steinbrenner, J., Beynon, J., Alfano, J.  2013.  The Pseudomonas syringae type III effector HopD1 suppresses effector‐triggered immunity, localizes to the endoplasmic reticulum, and targets the Arabidopsis transcription factor NTL9.  New Phytologist 201 (4) 1358–1370.

Garcia-Garcia A1, Anandhan A, Burns M, Chen H, Zhou Y, Franco R. Impairment of Atg5-dependent autophagic flux promotes paraquat- and MPP⁺-induced apoptosis but not rotenone or 6-hydroxydopamine toxicity. Toxicol Sci. 2013 Nov;136(1):166-82.

Wang G, Xu Z, Chen Z, Niu W, Zhou Y, Guo J, Tan L. 2013. Sequential binding of large molecules to hairy MOFs. Chem Commun (Camb). 2013 Jun 27; 49(59):6641-3.

Block, A., Fristedt, R., Rogers, S., Kumar, J., Barnes, B., Barnes, J., Elowsky, C., Wamboldt, Y., Mackenzie, S., Redding, K., Merchant, S., Basset, G.  2013.  Functional modeling identifies paralogous solanesyl-disphosphate synthases that assemble the side chain of plastoquinone-9 in plastids.  J. Biol. Chem 288 27594-27606.

Rodriguez-Rocha H, Garcia-Garcia A, Pickett C, Sumin L, Jones J, Chen H, Webb B, Choi J, Zhou Y, Zimmerman MC, Franco R. 2013. Compartmentalized oxidative stress in dopaminergic cell death induced by pesticides and complex I inhibitors: Distinct roles of superoxide anion and superoxide dismutases.  Free Radic Biol Med. 2013 Apr 19. pii: S0891-5849(13)00161-5. 2013.04.021. 

Shibata A, McMullen E, Pham A, Belshan M, Sanford B, Zhou Y, Goede M, Date AA, Destache C. 2013. Polymeric nanoparticles containing combination antiretroviral drugs for HIV-1 treatment. AIDS Res Hum Retroviruses. 2013 May; 29(5):746-54.

Maldonado, MX., T. Fangman, AF Pinto, JH Rupnow, RW Hutkins.  Inhibition of Lectin Adherence to Tissue Culture Cells by Prebiotic Carbohydrates.  Advances in Bioscience and Biotechnology: 2013, 4, 67-74

Elowsky, C., Jordan-Thaden, I., Kaul, R.  2013 A morphological analysis of a hybrid warm of native Ulmus rubra and introduced U. pumila (Ulmaceae) in Southeastern Nebraska.  Phytoneuron 44 1–23.

Valente RM, Ehlers E, Xu D, Ahmad H, Steadman A, Blasnitz L, Zhou Y, Kastanek L, Meng B, Zhang L. 2012 Toll-like receptor 7 stimulates the expression of epstein-barr virus latent membrane protein 1. PLoS One. 2012; 7(8): e43317.

Kumar T, Dweikat I, Sato S, Ge Z, Nersesian N, Chen H, Elthon T, Bean S, Loerger BP, Tilley M, Clemente T. Modulation of kernel storage proteins in grain sorghum (Sorghum bicolor (L.) Moench). Plant Biotechnol J. 2012 Jun;10(5):533-44.

Wu Y, Chen Q, Peng H, Dou H, Zhou Y, Huang Y, Zheng JC. 2012. Directed migration of human neural progenitor cells to interleukin-1β is promoted by chemokines stromal cell-derived factor-1 and monocyte chemotactic factor-1 in mouse brains. Transl Neurodegener. 2012 Jul 31; 1(1):15.

Lawrence N, Wells-Kingsbury J, Ihrig M, Fangman T, Namavar F, and Cheung C.  Controlling Bacterial Adhesion on Bioceramic Films Using Poly(Amino Acids) . ACS Langmuir, February 2012.

Ducluzeau, A., Wamboldt, Y., Elowsky, C., Mackenzie, S., Schuurink, R., Basset, G. 2012.  Gene network reconstruction identifies the authentic trans-prenyl diphosphate synthase that makes the solanesyl moiety of ubiquinone-9 in Arabidopsis. The Plant Journal 69 (2) 366–375.

Widhalm, j., Decluzeau, A., Buller, N., Elowsky, C., Olsen, L., Basset, G.  2012  Phylloquinone (vitamin k1) biosynthesis in plants: two two peroxisomal thioesterases of lactobacillales origin hydrolyze 1,4-dihydroxy-2-naphthoyl-coa.  The Plant Journal 71 (2) 205–215.

Kanmogne, GD, S. Singh, U. Roy, X. Liu, J. McMillan, S. Gorantla, S. Balkundi, N. Smith, Y. Alnouti, N. Gautam, Y. Zhou, L. Poluektova, A. Kabanov, T. Bronich, and HE. Gendelman, 2012.  Mononuclear phagocyte intercellular crosstalk facilitates transmission of cell-targeted nanoformulated antiretroviral drugs to human brain endothelial cells.  Internat. J. Nanomed. May 2012 Vol 2012 (7): 2373-88 (DOI: http://dx.doi.org/10.2147/IJN.S29454).

Chen, Z.G., Z.P. Xu, M. Zhang, Y. Zhou, M. Liu, T. Patten, G.Y. Liu, H. Li, X.C. Zeng, L. Tan,  2012.  "Two-Dimensional Crystallization of Hexagonal Bilayer with Moiré Patterns".   J.  Phys. Chem. B:  2012 Apr 12;116(14):4363-9. Epub 2012 Mar 14 (PMID:22356348)

Huang, C.,  Hongxia Zhou, Jianbin Tong, Han Chen, Yong-Jian Liu, Dian Wang, Xiaotao Wei, Xu-Gang Xia. FUS Transgenic Rats Develop the Phenotypes of Amyotrophic Lateral Sclerosis and Frontotemporal Lobar Degeneration. PLoS Genetics 2011 7(3): e1002011

Malinouski, M., Y. Zhou, V.V. Belousov, D.L. Hatfield, V.N. Gladyshev, 2011.  Hydrogen peroxide probes directed to different cellular compartments.  PLoS One. 2011 Jan 21; 6(1):e14564. 

Xu, Y., Arrieta-Montiel M., Wamboldt Y., Virdi K., dePaula, W., Widhalm J., Basset, G., Davila, J., Elthon, T., Elowsky, C., Sato, S., Clemente, T., Mackenzie S. 2011. MutS HOMOLOG1 is a Nucleoid Protein that alters mitochondrial and plastic properties and plant response to high light.  The Plant Cell 23:9, 3428–3441.

Balkundi S, Nowacek AS, Veerubhotla RS, Chen H, Martinez-Skinner A, Roy U, Mosley RL, Kanmogne G, Liu X, Kabanov AV, Bronich T, McMillan J, Gendelman HE. 2011. Comparative manufacture and cell-based delivery of antiretroviral nanoformulations.  Int J Nanomedicine. 2011;6: 3393-404.

Khanobporn, K., Chen Han, Baxendale Fred, Brewer Gary, Zhu Junwei 2011. Characterization of Olfactory Sensilla of Stomoxys calcitrans and Electrophysiological Responses to Odorant Compounds Associated with their Host and Oviposition. Medical and Veterinary Entomology. 2011 25; 327–336

Sarath G, Dien B, Saathoff AJ, Vogel KP, Mitchell RB, Chen H. 2011. Ethanol yields and cell wall properties in divergently bred switchgrass genotypes. Bioresour Technol. 2011 Oct;102(20):9579-85.

Quintero M, Maldonado M, Perez-Munoz, Jimenez R, Fangman T, Rupnow J, Wittke A, Russell M,  and Hutkins R.  2011. Adherence inhibition of Cronobacter sakazakii to Intestinal Epithelial Cells by Prebiotic Oligosaccharides.  Curr Microbiol, 2011 May: 62(5):1448-54,

Jiang S, Chen H, Wang Z, Riethoven JJ, Xia Y, Miner J, Fromm M. Activated AMPK and prostaglandins are involved in the response to conjugated linoleic acid and are sufficient to cause lipid reductions in adipocytes. J Nutr Biochem. 2011. Jul;22(7):656-64.

2006-2010

Wen HJ, Yang Z, Zhou Y, Wood C. 2010 Enhancement of autophagy during lytic replication by the Kaposi's sarcoma-associated herpesvirus replication and transcription activator. J Virol. 2010 Aug; 84(15):7448-58. 

Santo, C., Ee, W., Elowsky, C., Quaranta, D., Domaille, D., Christopher C., Grass, G.  2010.  Bacterial Killing by dry metallic copper surfaces.  Applied & Environmental Microbiology (77) 3, 794–802.

van Dijk K, Ding Y, Malkaram S, Riethoven JJ, Liu R, Yang J, Laczko P, Chen H, Xia Y, Ladunga I, Avramova Z, Fromm M. Dynamic changes in genome-wide histone H3 lysine 4 methylation patterns in response to dehydration stress in Arabidopsis thaliana. BMC Plant Biol. 2010 Nov 5;10:238.

Quarana, D., Krans, T., Santo, C., Elowsky, C., Domaille, D., Christopher C., Grass, G.  2010.  Mechanisms of contact-mediated killing of yeast cells on dry metallic copper surfaces.  Applied & Environmental Microbiology (77)2, 416–426.

Russo, S., Cannon, W., Elowsky, C., Tan, S., Davies, S.  2010.  Variation in leaf stomatal traits of 28 tree species in relation to water-use efficiency along an edaphic gradient in Bornean rain forest. American Journal of Botany (97)7, 1109–1120.

Zhou, H., Cao Huang, Han Chen, Dian Wang, Carlisle P. Landel, Pedro Yuxing Xia, Robert Bowser, Yong-Jian Liu, Xu Gang Xia. Transgenic Rat Model of Neurodegeneration Caused by Mutation in the TDP Gene. PLoS Genetics 2010 6(3): e1000887

Block, A., Guo, M., Li,G., Elowsky, C., Clemente, TE., Alfano, JR.  2010. The Pseudomonas syringae type III effector HopG1 targets mitochondria, alters plant development and suppresses plant innate immunity. Cell Microbiology 12(3), 318–330.

Kehr S, Malinouski M, Finney L, Vogt S, Labunskyy VM, Kasaikina MV, Carlson BA, Zhou Y, Hatfield DL, Gladyshev VN. 2009.  X-Ray Fluorescence Microscopy Reveals the Role of Selenium in Spermatogenesis.  J Mol Biol. 2009 Jun 26;389 (5):808-18.

Saira K, Zhou Y, Jones C. 2009.  The infected cell protein 0 encoded by bovine herpesvirus 1 (bICP0) associates with interferon regulatory factor 7 (IRF7), and consequently inhibits beta interferon promoter activity. J Virol. 2009 Apr;83 (8):3977-81.

Bharadwaj, A., Kovar, J., Loghman, E., Elowsky, C., Oakley, G., Simpson, M.  2009.  Spontaneous metastasis of prostate cancer if promoted by excess hyluronan synthesis and processing.  American Journal of Pathology 174(3), 1027–1036.

Dien, B.S.,  Gautam Sarath, Jeffrey F. Pedersen, Scott E. Sattler, Han Chen, Deanna L. Funnell-Harris, Nancy N. Nichols, Michael A. Cotta. Improved Sugar Conversion and Ethanol Yield for Forage Sorghum (Sorghum bicolor L. Moench) Lines with Reduced Lignin Contents. Bioenerg. Res. 2009 2:153–164

Wamboldt, Y., Mohammed, S., Elowsky, C., Wittgren, C., de Paula, W., Mackenzie, S.  2009.  Participation of leaky ribosomes scanning in protein dual targeting by alternative translation initiation in higher plants.  Plant Cell 21(1), 157–167.

Peng H, Whitney N, Wu Y, Tian C, Dou H, Zhou Y, Zheng J. 2008. HIV-1-infected and/or immune-activated macrophage-secreted TNF-alpha affects human fetal cortical neural progenitor cell proliferation and differentiation. Glia. 2008 Jun; 56(8):903-16.

Rogers AJ, Loggen M, Lee K, Angeletti PC. Varying efficiency of long-term replication of papillomaviruses in Saccharomyces cerevisiae. Virology. 2008 Nov 10;381(1):6-10.

Erume J, Berberov EM, Kachman SD, Scott MA, Zhou Y, Francis DH, Moxley RA. 2008. Comparison of the Contributions of Heat-Labile Enterotoxin and Heat-Stable Enterotoxin b to the Virulence of Enterotoxigenic Escherichia coli in F4ac Receptor-Positive Young Pigs. Infect Immun. 2008 Jul; 76(7):3141-9.

Saleh A, Alvarez-Venegas R, Yilmaz M, Le O, Hou G, Sadder M, Al-Abdallat A, Xia Y, Lu G, Ladunga I, Avramova Z. The highly similar Arabidopsis homologs of trithorax ATX1 and ATX2 encode proteins with divergent biochemical functions. Plant Cell. 2008 Mar;20(3):568-79.

Zhang, Y., Y.  Zhou, U. Schweizer, N. E. Savaskan, D. Hua, J. Kipnis, D. L. Hatfield, V. N. Gladyshev 2008. Comparative analysis of selenocysteine machinery and selenoprotein gene expression in mouse brain identifies key brain areas dependent on selenium.  J. Biol. Chem.283: 2427-38.

Chen, H., J. L. Frey, Tara Nazarenus, Christian Elowsky, Julie Stone. Ultrastructural Alterations in Arabidopsis thaliana dj1c Null Mutant Cotyledons. Microsc Microanal 2008; 14:(Suppl 2)

Lee, S.Y., Han Chen, Milford Hanna. Preparation and characterization of tapioca starch-poly(lactic acid) nanocomposite foams by melt intercalation based on clay type. J. of Industrial Crops and Products. Industrial Crops and Products. 2008; 28: 95–106

LaRosa, P.C., J. M. Riethoven, H. Chen, Y. Xia, Y. Zhou, M. Chen, J. L. Miner, and M. E. Fromm. 2007. Trans-10, Cis-12 Conjugated Linoleic Acid Activates the Integrated Stress Response Pathway in Adipocytes.  Physiol. Genomics Nov 14; 31(3):544-53

Sarath G, Hou G, Baird LM, Mitchell RB. Reactive oxygen species, ABA and nitric oxide interactions on the germination of warm-season C4-grasses. Planta. 2007 Aug; 226(3):697-708.

Meyer F, Perez S, Jiang Y, Zhou Y, Henderson G, Jones C. 2007. Identification of a novel protein encoded by the latency-related gene of bovine herpesvirus 1. J Neurovirol. 2007 Dec;13(6):569-78

Chen, K.C, Y. Zhou, W. Zhang, M.F. Lou. 2007. Control of PDGF-induced reactive oxygen species (ROS) generation and signal transduction in human lens epithelial cells.  Mol. Vis. 13:374-87.

Sarath G, Hou G, Baird LM, Mitchell RB. ABA, ROS and NO are Key Players During Switchgrass Seed Germination. Plant Signal Behav. 2007 Nov;2(6):492-3.

Dumitru, R., Navarathna, D., Semighini, C., Elowsky, C., Dumitru, R., Dignard, D., Whiteway, M., Atkin, A., Nickerson, K.  2007.  In vivo and in vitro anaerobic mating in Candida albicans.   Eukaryotic Cell 6(2), 465–472.

Saira K, Y. Zhou, C. Jones 2007. The infected cell protein 0 encoded by bovine herpesvirus 1 (bICP0) induces degradation of interferon response factor 3 (IRF3), and consequently inhibits beta interferon promoter activity. J. Virol. 81(7):3077-86.

Dou, H., J. Morehead,  C. J. Destache, J. Limoges, B. Ellison, J. D. Kingsley, L.  Shlyakhtenko, Y. Zhou, M. Chaubal,  J.  Werling,  J. Kipp,  B. Rabinow, and H. E. Gendelman. 2007. Laboratory investigations for the morphologic, pharmacokinetic, and anti-retroviral properties of indinavir nanoparticles in human monocyte-derived macrophages.  Virol. 358(1):148-58.

Kabil, O., Y. Zhou and R. Banerjee. 2006. Human cystathionine β-synthase is a target for sumoylation.  Biochem. Nov 14;45(45):13528-36

LaRosa, P.C., J. Miner, Y. Xia, Y. Zhou, S. Kachman and M. E. Fromm.  2006 Trans-10, cis-12 conjugated linoleic acid causes inflammation and delipidation of white adipose tissue in mice: a microarray and histological analysis. Physiol. Genomics Nov 27;27(3):282-94.

Perez S, Lovato L, Zhou J(Y), Doster A, Jones C. 2006. Comparison of inflammatory infiltrates in trigeminal ganglia of cattle infected with wild-type Bovine herpesvirus 1 versus a virus strain containing a mutation in the LR (latency-related) gene.  J Neurovirol. 2006 Oct;12(5):392-7.

Das, S.C., D. Nayak, Y. Zhou, and A. K. Pattnaik.  2006.  Visualization of intracellular transport of vesicular stomatitis virus nucleocapsids in living cells.  J. Virol. 80: 6368-6377.

Zhang, Y, Jiang Y, Geiser V, Zhou J(Y), Jones C. 2006. Bovine herpesvirus 1 immediate-early protein (bICP0) interacts with the histone acetyltransferase p300, which stimulates productive infection and gC promoter activity. J. Gen. Virol. 87:1843-51.

Sarath G, Bethke PC, Jones R, Baird LM, Hou G, Mitchell RB. Nitric oxide accelerates seed germination in warm-season grasses. Planta. 2006 May;223(6):1154-64.

Chowdhury, S.I.  Al-Mubarak, A., Y. Zhou. 2006. The Us9 gene of bovine herpesvirus 1 (BHV-1) effectively complements a Us9-null strain of BHV-5 for anterograde transport, neurovirulence, and neuroinvasiveness in a rabbit model.  J. Virol. 80:4396-405.

2001-2005

Koka S, Minick GT, Zhou Y, Westendorf JJ, Boehm MB. 2005. Src regulates the activity of the mammalian formin protein FHOD1. Biochem Biophys Res Commun. 336(4): 1285-91.

Qu F, Ye X, Hou G, Sato S, Clemente TE, Morris TJ. RDR6 has a broad-spectrum but temperature-dependent antiviral defense role in Nicotiana benthamiana. J Virol. 2005 Dec;79(24):15209-17.

Boehm, M.B., T.J. Milius, Y.  Zhou, J. J. Westendorf, S. Koka, 2005. The mammalian formin FHOD1 interacts with the ERK MAP kinase pathway. Biochem. Biophys. Res. Commun. 335(4):1090-4.

Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN. 2005. Mammalian selenoprotein thioredoxin-glutathione reductase. Roles in disulfide bond formation and sperm maturation. J. Biol Chem. 280(28):26491-8.

Zhang Y, Zhou J(Y), Jones C. 2005 Identification of functional domains within the bICP0 protein encoded by bovine herpesvirus 1. J Gen Virol. 86:879-86.

Dassanayake, R.P., Y.  Zhou, S. Hinkley, C.J. Stryker, G. Plauche, J.T. Borda,  K. Sestak,  and G.E. Duhamel.  2005. Characterization of cytolethal distending toxin of campylobacter species isolated from captive macaque monkeys.  J. Clin.  Microbiol. J Clin Microbiol. 43(2):641-9.

Heckman, N., Roch, G., Horst, G., Elowsky, C. 2005.  Growth regulator effects on cellular characteristics of two turfgrass species.  International Turfgrass Society Research Journal 10, 857–861.

Rhoads, D. M., S. J. White, Y. Zhou, M. Muralidharan, T. E. Elthon. 2005. Altered gene expression in plants with constitutive expression of mitochondrial small heat shock protein suggests the involvement of retrograde regulation in the heat stress response. Physiol. Plantarum 123:435–444.

Richard, D.S., R. Rybczynski, T.G. Wilson, Y. Wang, M.L. Wayne, Y.  Zhou, L. Partridge and L.G. Harshman. 2005.  Insulin signaling is necessary for vitellogenesis in Drosophila melanogaster independent of the roles of juvenile hormone and ecdysteroids:  female sterility of the chico insulin signaling mutation is autonomous to the ovary. J. Insect Physiol. 51:1455-464.   

Liu, Y., Z. Liu, H. Gao, Y. Zhou, E. J. Androphy and J. J. Chen. 2005 Opposing Effects of Bovine Papillomavirus Type 1 E6 and E7 Genes on Fas-Mediated Apoptosis. Oncogene 24(24):3942-53

Christensen, C., Lyznik A., Mohammed, S., Elowsky, C., Elo A., Yule R., Mackenzie S.  2005.   Dual-domain, dual-targeting organellar protein presequences in Arabidopsis can use non-AUG start codons.  Plant Cell 17(10), 2805–2816.

Zhang L, Zhang J, Lambert Q, Der CJ, Del Valle L, Miklossy J, Khalili K, Zhou Y, Pagano JS. 2004 Interferon regulatory factor 7 is associated with Epstein-Barr virus-transformed central nervous system lymphoma and has oncogenic properties. J Virol. 78(23):12987-95.

Vitvitsky V, S. Dayal, S. Stabler, Y. Zhou, H. Wang, S.R. Lentz, R. Banerjee.  2004. Perturbations in homocysteine-linked redox homeostasis in a murine model for hyperhomocysteinemia. Am J Physiol. Regul. Integr. Comp. Physiol.. 287(1):R39-46. 

Inman M, J(Y). Zhou, H. Webb, C. Jones. 2004. Identification of a novel bovine herpesvirus 1 transcript containing a small open reading frame that is expressed in trigeminal ganglia of latently infected cattle. J. Virol. 78 (10):5438-47.

Dasgupta S, M. Jana M, Y. Zhou, Y.K. Fung, S. Ghosh, K.  Pahan. 2004 Antineuro-inflammatory effect of NF-kappa-B essential modifier-binding domain peptides in the adoptive transfer model of experimental allergic encephalomyelitis. J. Immunol. 173(2):1344-54.

Berberov, E.M.  Y. Zhou, D. H. Francis, M.A. Scott, S.D. Kachman, and R.A. Moxley 2004. Relative importance of heat-labile enterotoxin in the causation of severe diarrheal disease in the gnotobiotic piglet model by a strain of enterotoxigenic Escherichia coli that produces multiple enterotoxins.  Infect Immun. 72(7):3914-24.

Ross, E., Stone, J., Elowsky, C., Arredondo-Peter, R., Klucas, R., Sarath, G.  2004.  Activation of the Oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, AAR1.  Journal of Experimental Botany 55, 1721–1731.

Al-Mubarak, A., Y. Zhou, S.I. Chowdhury 2004.  A glycine-rich BHV-5 gE-specific epitope within the ectodomain is important for BHV-5 neurovirulence.  J Virol. 78:4806-16

Chen, K. C-W. Y. Zhou, K. Xing, K. Krysan and M.F. Lou 2004.  Platelet Derived Growth Factor (PDGF)-induced Reactive Oxygen Species in the Lens Epithelial Cells: The Redox Signaling. Exp. Eye Res. 78:1057-1067.

Xu, W., Y. Zhou, R. Chollet. 2003. Identification and expression of a soybean nodule-enhanced PEP-carboxylase kinase gene (NE-PpcK) that shows striking up-/down-regulation in vivo.  Plant J. 34(4):441-52.

Ryan, L., Peng, H., Erichsen, D., Huang, Y., Persidsky, Y. Zhou, H.E.  Gendelman, and J. Zheng. 2004. Links between Tumor Necrosis Factor Related Apoptosis Inducing Ligand Mediated Human Neuronal Apoptosis and HIV-1 Associated Dementia. J. Neuroimmunol. 148: 127-139.

Dasgupta S., Y. Zhou, M. Jana, N.L. Banik and K. Pahan. 2003.  Sodium phenylacetate inhibits the adoptive transfer of experimental allergic encephalomyelitis in SJL/J mice at multiple steps.  J. Immunol. 170(7):3874-82.

Heng-Moss TM, Baxendale FP, Riordan TP, Young L, Lee K. Chinch bug-resistant buffalograss: an investigation of tolerance, antixenosis, and antibiosis. J Econ Entomol. 2003 Dec;96(6):1942-51.

Vaidyanathan, H., Y. Zhou, T. M. Petro and S.D. Schwartzbach. 2003.  Intracellular localization of the p35 subunit of murine IL-12.  Cytokine Feb 7;21(3):120-128.

Komina, O., Y. Zhou, G. Sarath, R. Chollet. 2002.  In Vivo and In Vitro Phosphorylation of Membrane and Soluble Forms of Soybean Nodule Sucrose Synthase (Nodulin-100).  Plant Physiol. 129(4):1664-1673.

Buhr, T.,  S. Sato, F. Ebrahim, A. Xing, Y. Zhou, M. Mathiesen, B. Sweiger, A. Kinney, P. Staswick, and T. Clemente. 2002. Ribozyme termination of RNA transcripts down-regulate seed fatty acid genes in transgenic soybean.  Plant J. 30(2):155-163.

Chowdhury, S.I., P.S. Bhattachajee, A. Al-Mubarak, M.L. Weiss, M. Onderci, and Y. Zhou. 2002. Bovine Herpesvirus 5 (BHV-5) Us9 Is Essential for BHV-5 Neuropathogenesis. J. Virol. 76 (8):3839-51.

Datta R, P. Choudhury, M. Bhattacharya M, F. Soto Leon, Y. Zhou, B. Datta.  2001. Protection of translation initiation factor eIF2 phosphorylation correlates with eIF2-associated glycoprotein p67 levels and requires the lysine-rich domain I of p67.  Biochimie 83(10):919-931.

Ross, E.J.H, L. Shearman, M. Mathiesen, Y. Zhou, R.  Arredondo-Peter, G. Sarath, R. V. Klucas 2001.  Non-symbiotic hemoglobins in rice are expressed during germination and in differentiating cell types.  Protoplasma 218:125-133.

Li, L.J., K. Arumuganathan, H.W. Rines, R.L. Phillips, O. Riera-Lizarazu, D. Sandhu, Y. Zhou,  K.S. Gill,.  2001. Flow Cytometric sorting of maize chromosome 9 from an oat-maize addition line.  Theoretical Applied Genetics 102:658-663.

Korotkov, K.V., E. Kumaraswamy, Y. Zhou, D.L. Hatfield and V. N. Gladyshev. 2001. Association between the 15 kDa selenoprotein and UDP glucose:glycoprotein glucosyltransferase in the endoplasmic reticulum of mammalian cells.  J. Biol. Chem. 276:15330-15336.

Advisory Committee

Facility Advisory Group
The mission of the Facility Advisory Group is to provide guidance and feedback to the facility regarding the most effective use of its resources to meet research demands.

James R. Alfano, PhD
Phone: (402) 472-0395
Email: jalfano2@unl.edu
Alfano Lab website

Oleh Khalimonchuk, PhD
Phone: (402) 472-8060
Email: okhalimonchuk2@unl.edu
Khalimonchuk Lab website

Yongfeng Lu, Ph.D.
Phone: 402-472-8323
Email: ylu2@unl.edu
Visit Faculty Webpage

Matthew Wiebe, Ph.D.
Phone: (402) 472-4154
Email: mwiebe2@unl.edu
Visit Faculty Webpage

Contact the Director

Director: You "Joe" Zhou, PhD
E119.5 Beadle Center

Telephone Number:
402-472-5935 (Office)
402-472-5942 (Core Facility)
Email: yzhou2@unl.edu