Morrison Microscopy Core Research Facility
Located on the first floor of the George W. Beadle building, the Morrison Microscopy Core Research Facility has state of the art imaging systems including light/fluorescence microscopes, confocal laser scanning microscopes and electron microscopes. The microscopy core facility will provide strong technical support for multi-disciplinary research in the state of Nebraska. We will also provide services, teaching and training for university research communities.
Supported by: 1) Nebraska State funding by the Nebraska Research Initiative; 2) Federal funding by NIH COBREs (NCV and Redox); 3) A private donation from the Ken Morrison Family; 4) NU Foundation; and 5) usage/service fees.
New user to this core?
If you are new to the Microscopy core and need to use one of the image tools of the core, please fill out the Request for Microscopy Services form and send it with your email to Dr. Y. Joe Zhou or one of other microscopy specialists of the core (See Personnel tab). In order for us to assist you efficiently, we need to have some background information about your research project(s) related to microscopy so that we can identify which imaging system is the best fit for your experiment and what kind of training is needed.Request for Microscopy Services Form
We will follow up your request and schedule an appointment with a core specialist to discuss some specifics on sample preparation and imaging collections in the Microscopy core.
Note: Up to 4 hrs at assisted rate will be applied to all new users (training fee included).
Click here for printable copy of fees.
|Service||Academic/In House||Industry/Private Company Users|
|TEM/SEM - Assisted||$50/hour||$150/hour (Assisted)|
|TEM - Unassisted||$35/hour||$100/hour|
|Laser Capture Micro-dissection System||$15/hour plus the cost for disposable materials||$40/hour plus the cost for disposable materials|
|SEM Sample Preparation||$20/sample||$60/sample|
|TEM Negative Stain||$26/sample||$70/sample|
|Standard TEM Preparation (fix, embed, section and stain)||$53/sample ($10 for each additional TEM grid)||$120/ first sample ($30 for each additional TEM grid)|
|Additional sectioning/straining of the embedded samples||$27/sample||$60/sample|
|Frozen and paraffin Sectioning by user||$30/sample plus material cost
$30/use (up to 4 hrs)
|Sputter Coating||$20/run (Free when examining the samples using this core’s SEMs)||$55/run|
Instruments & Facilities
Advanced Fluorescence Microscopes
The inverted (Olympus IX-81) confocal microscopes use confocal optics for high resolution, high contrast and increased resolution in the light axis direction. Acquisition functions include 3D construction, Z series sectioning, time series observations, sequential laser scan and image analysis functions. A total of 5 channels can be acquired simultaneously with 4 fluorescence images and a transmitted light image. The confocal has 6 laser lines (405, 458, 488, 514, 543, and 633 nm) and can be used for multiple fluorescence labeling detection.
The Nikon A1 confocal system on a Nikon 90i upright fluorescence microscope can provide 6 different excitation laser lines, 405, 457, 476, 488, 514, 561, and 641nm. It is a standard four-channel confocal imaging system but also has a spectral detector allowing for 10 nm, 6 nm and 2.5 nm resolution of emission spectra across up to 32 channels.
Nikon Ti-S Inverted Fluorescence Microscope with Digital camera allows researchers to collect images under sterile conditions.
Laser Capture Microdissection system (LCM, PixCell IIe from Arcturus) provides one of the best tools available to date for isolation of individual cells or specific populations of cells, or bacteria of interest from tissue sections for further molecular and biochemical analyses.
Upright fluorescence microscope (Olympus AX70) with digital camera.
Stereo fluorescence microscope (Nikon SMZ800) with digital camera that allows users to examine samples under sterile conditions for phenotypic selections.
EVOS® FL Auto Cell Imaging System with an automated microscopic stage (computer controlled X-Y-Z movement), dual cameras, selectable excitation/emission filters from 5 LED Light Cubes (DAPI, GFP, RFP, Texas Red, and Cy5), and 3x optical zoom over different lenses (4x, 10x, 20x, 40x, 60x, and 100x). This system is user friendly and suitable for single or multi-labeling fluorescence or colorimetric image acquisitions from sections or live cells using single culture dish, 6, 12, or 24-well culture plates, or 96 well ELISA plate.
Hitachi H7500 TEM is an advanced electron microscope with a window-based computerized operating system for a) ultrastructural analysis on ultrathin sections of samples, b) assay of nanoparticles, and c) examination of negative stained microbial particles (such as virus) and nanoparticles. A bottom-mount high-resolution CCD camera is installed with this system.
Magnification range: 50x to 200,000x (HC mode at 80 KV); Resolution (point to point): up to 0.5 nm
Hitachi S4700 Field-Emission SEM is a powerful tool for topographic analysis at nano-scale levels, which uses windows-based computerized operating system with the high-resolution digital processing capacity.
Magnification range: 20x to 500,000x; Resolution: up to 1.0 nm
Hitachi S-3000N Variable-Pressure SEM is an advanced electron microscopic imaging system with the capability of using high vacuum or a variable pressure range from 1-270 Pa. This SEM uses a Windows -based computerized operating system with high-resolution digital processing capacity and is most suitable for topographic analysis using biological or geological samples.
Magnification range: 25x to 200,000x; Resolution: up to 3 nm in high vacuum mode and up to 5 nm in variable pressure mode
- Determination of cell viability or cell survival
- In situ hybridization
- Co-localization of GFP-tagged proteins with markers of cellular components and live cell imaging
- Whole-mount immunochemical fluorescence confocal microscopy
- Immunogold labeling method for electron microscopy (for ultrathin sections)
- Immunofluorescence microscopy using frozen sections, chamber-slides or coverslips
- TEM buffers fixation methods for general users
- Chart for laser lines and filters for FV500 and A1 confocal systems
Facility Advisory Group
The mission of the Facility Advisory Group is to provide guidance and feedback to the facility regarding the most effective use of its resources to meet research demands.
Yongfeng Lu, Ph.D.
Visit Faculty Webpage
Clinton Jones, Ph.D.
Phone: (402) 472-1890
Visit Faculty Webpage
James R. Alfano, PhD
Phone: (402) 472-0395
Alfano Lab website