Morrison Microscopy Core Research Facility
Located on the first floor of the George W. Beadle building, the Morrison Microscopy Core Research Facility has state of the art imaging systems including light/fluorescence microscopes, confocal laser scanning microscopes and electron microscopes. The microscopy core facility will provide strong technical support for multi-disciplinary research in the state of Nebraska. We will also provide services, teaching and training for university research communities.
Supported by: 1) Nebraska State funding by the Nebraska Research Initiative; 2) Federal funding by NIH COBREs (NCV and Redox); 3) A one-time private donation from the Ken Morrison Family through NU Foundation; and 4) usage/service fees.
|Service||Academic/In House||Industry/Private Company Users|
|TEM/SEM - Assisted||$60/hour||$250/hour (Assisted)|
|TEM - Unassisted||$35/hour||– –|
|Shotgun Library Prep||$75.00 per sample||$75.00 per sample|
|Laser Capture Micro-dissection System||$65/hour plus the cost for disposable||$150/hour plus the cost for disposable materials|
|SEM Sample Preparation||$20/sample||$63/sample|
|TEM Negative Stain||$26/sample||$75/sample|
|Standard TEM Preparation (fix, embed, section and stain)||$53/sample ($10 for each additional TEM grid)||$150/ first sample ($30 for each additional TEM grid)|
|Additional sectioning/straining of the embedded samples||$27/sample||$75/sample|
|Frozen and paraffin Sectioning By user||
$30/sample plus material cost
$30/use (up to 4 hrs)
|Sputter Coating||$20/run (Free when examining the samples using this core’s SEMs)||$55/run|
Instruments & Facilities
Advanced Fluorescence Microscopes
The inverted (Olympus IX-81) confocal microscopes use confocal optics for high resolution, high contrast and increased resolution in the light axis direction. Acquisition functions include 3D construction, Z series sectioning, time series observations, sequential laser scan and image analysis functions. A total of 5 channels can be acquired simultaneously with 4 fluorescence images and a transmitted light image. The confocal has 6 laser lines (405, 458, 488, 514, 543, and 633 nm) and can be used for multiple fluorescence labeling detection.
The Nikon A1 confocal system on a Nikon 90i upright fluorescence microscope can provide 6 different excitation laser lines, 405, 457, 476, 488, 514, 561, and 641nm. It is a standard four-channel confocal imaging system but also has a spectral detector allowing for 10 nm, 6 nm and 2.5 nm resolution of emission spectra across up to 32 channels.
Nikon Ti-S Inverted Fluorescence Microscope with Digital camera allows researchers to collect images under sterile conditions.
Laser Capture Microdissection system (LCM, PixCell IIe from Arcturus) provides one of the best tools available to date for isolation of individual cells or specific populations of cells, or bacteria of interest from tissue sections for further molecular and biochemical analyses.
Upright fluorescence microscope (Olympus AX70) with digital camera.
Stereo fluorescence microscope (Nikon SMZ800) with digital camera that allows users to examine samples under sterile conditions for phenotypic selections.
Hitachi H7500 TEM is an advanced electron microscope with a window-based computerized operating system for a) ultrastructural analysis on ultrathin sections of samples, b) assay of nanoparticles, and c) examination of negative stained microbial particles (such as virus) and nanoparticles. A bottom-mount high-resolution CCD camera is installed with this system.
Magnification range: 50x to 200,000x (HC mode at 80 KV); Resolution (point to point): up to 0.5 nm
Hitachi S4700 Field-Emission SEM is a powerful tool for topographic analysis at nano-scale levels, which uses windows-based computerized operating system with the high-resolution digital processing capacity.
Magnification range: 20x to 500,000x; Resolution: up to 1.0 nm
Hitachi S-3000N Variable-Pressure SEM is an advanced electron microscopic imaging system with the capability of using high vacuum or a variable pressure range from 1-270 Pa. This SEM uses a Windows -based computerized operating system with high-resolution digital processing capacity and is most suitable for topographic analysis using biological or geological samples.
Magnification range: 25x to 200,000x; Resolution: up to 3 nm in high vacuum mode and up to 5 nm in variable pressure mode
- Determination of cell viability or cell survival
- In situ hybridization
- Co-localization of GFP-tagged proteins with markers of cellular components and live cell imaging
- Whole-mount immunochemical fluorescence confocal microscopy
- Immunogold labeling method for electron microscopy (for ultrathin sections)
- Immunofluorescence microscopy using frozen sections, chamber-slides or coverslips
- TEM buffers fixation methods for general users
Facility Advisory Group
The mission of the Facility Advisory Group is to provide guidance and feedback to the facility regarding the most effective use of its resources to meet research demands.
Yongfeng Lu, Ph.D.
Visit Faculty Webpage
Clinton Jones, Ph.D.
Phone: (402) 472-1890
Visit Faculty Webpage
James R. Alfano, PhD
Phone: (402) 472-0395
Alfano Lab website