Genomics

GENOMICS
GENOMICS

Services

  • Ion Torrent Sequencing available at UNL
  • Illumina Next Gen Sequencing available through the University of Minnesota

Ion Torrent Sequencing Information

Ion Torrent PGM Sequencing and Library Preparation for 16S rRNA sequencing, and shotgun sequencing.

Ion Torrent™ PGM Applications

Targeted DNA Sequencing

  • 16S rRNA library preparation and sequencing with multiplexing ability for up to 96 samples
  • Current capability - 16S library preparation for bacteria V3 region and the V4-V5 for archaea
  • Cost of 16S library preparation, normalization and pooling at $ 6.25/sample when doing 96 samples
  • 3 sequencing options based on sequencing depth needed ranging from 500,000 reads to 4,000,000 reads
Small Genome Sequencing ( < 5Mb )
  • Sequence bacterial genomes with 20X-75X coverage adequate for genome assembly
  • Multiplex up to 24 samples (based on sequencing depth per sample)
  • Library preparation for sequencing at $ 75 per sample (adequate amount of good quality DNA must be provided)
  • 3 sequencing options based on sequencing depth needed ranging from 500,000 reads to 4,000,000 reads
Transcriptome sequencing and de novo sequencing
  • Sequence poly-A enriched or enriched bacterial mRNA samples
  • Multiplex up to 24 samples (based on sequencing depth per sample)
  • Library preparation for sequencing at $ 75 per sample after cDNA synthesis
  • 3 sequencing options based on sequencing depth needed ranging from 500,000 reads to 4,000,000 reads

Click image below for complete pricing information

University of Minnesota Contact Information

Service Fees for Ion Torrent Sequencing

Shotgun Library Prep $75.00 per sample
16S Library Prep $600.00 per sample
314 Chip - 200 bp $650.00 per chip
316 Chip - 200 bp $850.00 per chip
318 Chip - 200 bp $1050.00 per chip
314 Chip - 400 bp $800.00 per chip
316 Chip - 400 bp $1000.00 per sample
318 Chip - 400 bp $1200.00 per sample
DNA Extractino $10.00 per sample

Instruments & Facilities

  • Ion Torrent PGM
  • One Touch 2
  • Bioanalyzer
  • Pippin prep
  • epMotion 5070 liquid handler
  • Bioruptor sonicator

Personnel

Andrea McCain, Technician
ANS C220
Phone: 402-472-6486
Email Andrea

Contact the Manager

Manager: Samodha Fernando, PhD
Assistant Professor
Dept of Animal Science
C220K
Lincoln, NE 68588-0304

Ph: (402) 472-0518
Email: sfernando2@unl.edu