Flow Cytometry

Flow Cytometry
FLOW CYTOMETRY

Services

Located in the Morrison Center, the Flow Cytometry core provides comprehensive data collection, data interpretation and education in flow cytometry.

Hours: Monday-Friday 9:00 am-5:00 pm or by appointment

Service Fees

Internal Users - All instruments: $30/hour with a ½ hour minimum

External Users – All Instruments: $50/hour with a ½ hour minimum

Additional $50 setup fee for use of the Aria

Policies

Late Cancellations – Users who have scheduled time may cancel up to 24 hours in advance of their start time. If users do not show up for a scheduled appointment, or cancel with 24 hours of the appointment, they will be billed for the scheduled amount of time.

Training – All new users will participate in one or more training sessions with Service Center staff. New users within an experienced lab may be instructed by trained personnel in the lab, but prior to being able to run samples by themselves, they will also be required to participate in a training session with Service Center staff.

After hours / Weekend Use – The Service Center is open Monday – Friday, from 8am – 5pm. Trained users may be allowed access to the facility after hours and on weekends, after they have been given approval from the Service Center Manager. Please inform Manager of any plans to work outside of normal lab hours.

Radioactive Materials – Radiolabeled samples are not permitted in the Service Center.

Acknowledgements – Please acknowledge the Flow Cytometry Service Center in any grant application or publication in which equipment and/or staff expertise was provided. Also, please send the Service Center manager a copy of accepted publications containing data generated in the facility.

Biosafety – In an effort to decrease possible infection risk to Service Center staff and lab users, we require that all samples be fixed prior to analysis. We are aware that certain applications preclude the fixing of samples. Should there be experimental reasons that your protocol is not compatible with fixation, please discuss this with Service Center staff before running samples so appropriate handling and containment can be implemented.

Instruments

Make     |           Model                     |       Equipment Function
BD FACSCalibur

The Flow Cytometry Service Center is equipped with two FACSCaliburs. Both have two lasers (488 and 635nm), and are capable of detecting FSC, SSC and 4 colors. Both instruments operate under CellQuest Pro software.

Fluorofinder Panel Builder

FACSCalibur
BD FACS Array This is a Dual-Laser cytometer, with 532 and 635nm excitation, designed for screening applications, using cellular or bead-based assays. It is capable of four color analysis operating under FCAP and BD FACSArray System Software in a 96-well format.
FACSArray
Cytek DxP10

Our DxP10 is a BD FACSort that has been upgraded to a Four-laser cytometer (407, 488, 561, and 637nm). It is capable of detecting FSC, SSC and 10-colors. It operates under FlowJo CE.

Fluorofinder Panel Builder

Cytek
BD FACSAria II

The Service Center’s Aria is a four-laser platform (405, 488, 561 and 642nm) instrument capable of detecting FSC, SSC and 16-colors. It is the Center’s only instrument capable of sorting samples. It is able to sort up to four separate populations from the same sample, at a rate of up to 30,000 events/second. It is stationed in a Baker BioPROTECT biosafety cabinet. It operates under BD FACSDiva Software.

Fluorofinder Panel Builder

FACSAria

Instrument Reservations


Check the calendar below and then submit a request to use an instrument.

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Personnel

Dirk Anderson, Manager
MOLR 160
Office Phone: 402-472-3129
Lab Phone: 402-472-3142
Email Dirk

Advisory Committee

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Facility Homepage

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Contact the Director

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