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University of Nebraska–Lincoln

Center for Biotechnology

Protein

The Protein Core Facility was recently established to provide a resource to researchers that want to purify, separate, or identify proteins. In addition, we can help scientists solve problems that they have encountered in their protein research. The Protein Core Facility will develop to meet any capabilities needed in the expanding field of proteomics and is located in the Beadle Center room E240.

Gene expression services are now provided by the Protein Core Facility. These include bench-scale (analytical level) construction of microbial strains and compatible vectors for recombinant protein overproduction. Host organisms include E. coli (bacteria) and S. solfataricus (archaea). Fungal hosts (S. cereviseae and P. pastoris) and phage (lambda, M13) are available by request. All commercial protein affinity tags are available. Primer design and purchase is negotiable.

Manager

Dr. Tom Elthon
E243 Beadle Center
Tel: (402) 472-6245
Email: telthon1@unlnotes.unl.edu