Services
| The Protein Core Facility provides the following services: |
-
separation of proteins by one and two-dimensional gel electrophoresis
- analysis of two-dimensional gels
- western transfers and immunoblotting
- separation of proteins by column chromatography
ion exchange
hydrophobic interaction
affinity
- protein extraction from tissues that have been pulverized in liquid N2
- other procedures as requested
|
| Gene expression services include: |
- Plasmid vector construction (E. coli)
Affinity tagging
Site specific mutagenesis
- Strain Construction (E. coli and S. solfataricus)
Gene disruption
Gene deletion, substitution
- Protein overproduction (E. coli and S. solfataricus)
Fermentation validation
Codon/mRNA modification
Protein secretion, folding, refolding
|
