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University of Nebraska–Lincoln

Center for Biotechnology

Protein

Protocols

Gel Electrophoresis
Protein extraction from whole tissues for IEF
Precipitation of soluble proteins for IEF
IEF
SDS-PAGE
PAGE stains
Spot picking for MS/MS
Western transfer
Immunoblotting

Column Chromatography
His-tagged proteins
Ion exchange (HiTrap Q XL)
Hydrophobic interaction (HiTrap phenyl HP)
Desalting

Antibody purification
Gel filtration

Rotofor
Preparative IEF