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Gel Electrophoresis Protein extraction from whole tissues for IEF Precipitation of soluble proteins for IEF IEF SDS-PAGE PAGE stains Spot picking for MS/MS Western transfer Immunoblotting Column Chromatography His-tagged proteins Ion exchange (HiTrap Q XL) Hydrophobic interaction (HiTrap phenyl HP) Desalting Antibody purification Gel filtration Rotofor Preparative IEF