Plant Transformation Services
Transformations of the model crops, wheat and soybean will be carried out via Agrobacterium-mediated protocols. Preliminary screens will be conducted to verify transformation events, by monitoring for the expression and/or integration of the selectable marker. Presence of the desired transgene will be guaranteed within the lines delivered, however, co-expression of the trait can not be assured.
The customer may request the full service of the Facility, in which case we will initiate and maintain all the cultures and the client will be provided with the transformants. Clients wishing to conduct their own transformation, may utilize the laboratory and have all the necessary supplies and growth chamber space provided. In the latter case the Facility's staff will be available for assistance and consultations.
Agrobacterium-mediated transformation will be conducted utilizing established protocols for the model crop systems, Arabidopsis and Tobacco. For the former plant species, transformations may be conducted by either the root transformation or by the vacuum infiltration of whole plants.
Soybean transformations will utilize a direct organogenic regime for plantlet differentiation coupled with Agrobacterium-mediated transformation for gene delivery into the crop.
Wheat transformations will be carried out using Agrobacterium-mediated transformation protocol using immature embryos as the target tissue. The plantlet formation will arise via an embryogenic pathway from the cultured explants.
The client will provide the Agrobacterium culture carrying the desired cassettes. Plasmid rescue from Agrobacterium and conformation of the plasmid's integrity must be conducted prior to cultures being handed off to the Facility.
Preliminary expression data must be ascertained prior to the constructs being delivered into soybean or wheat. Preliminary expression data may include information obtained from stable expression within model crop systems and/or transient expression data collected from a protoplast system.
