Co-localization of GFP-tagged proteins with markers of cellular components and live cell imaging
- a) Dilute the marker (e.g., Lysotracker, mitotracker, or Golgi marker) in serum-free HEPES-MEM in a tube and mix it well. (1-2 uM final concentration; 1.5 - 2 ml per 35 mm dish)
b) Gently rinse the cells after transfection with serum-free HEPES-MEM and add the medium with the markers in to the plate carefully.
c) Incubate the culture for 20-25 min at room temperature.
d) Change the medium (rinse) the cells with 5 ml HEPES-MEM once to remove the dye solution. Add 6-7 ml of the same medium to the dish.
e) Examine the data under the confocal microscope and collect the images using the FITC/CY3/Phase program.
