Olympus FV500 Upright (Olympus BX60) and Inverted (Olympus IX 81)Confocal microscopes use confocal optics for high resolution, high contrast and increased resolution in the light axis direction. Acquisition functions include 3D construction, Z series sectioning, time series observations, sequential laser scan and image analysis functions. A total of 5 channels can be acquired simultaneously with 4 fluorescence images and a transmitted light image. The confocal has 6 laser lines (405, 458, 488, 514, 543, and 633 nm) and can be used for multiple fluorescence labeling detection.